GHRP-6

In animal studies

These are doses given to animals, usually per kilogram of body weight. They do not convert directly to a human dose.

• 0.4 mg/kg

  rats · for 7 days

  Growth Hormone-Releasing Peptide-6 (GHRP-6) Ameliorates Post-Infarct Ventricular Remodeling and Systolic Dysfunction in a Model of Permanent Coronary Ligation.Pharmaceuticals (Basel, Switzerland) · 2026

  “Having determined 0.4 mg/kg as the minimum effective dose for enhancing inotropy based on echocardiographic parameters in healthy rats, we implemented a non-reperfusion myocardial infarct model, with its consequent left ventricle wall thinning and ballooning, via permanent left descending coronary artery ligation.”

• See source wording

  rats · another route · twice a day · for 7 days

  Changes in Locomotor Activity Observed During Acute Nicotine Withdrawal Can Be Attenuated by Ghrelin and GHRP-6 in Rats.Biomedicines · 2025

  “Methods: Male and female Wistar rats were exposed daily to intraperitoneal (ip) injection with 2 mg/kg nicotine or saline solution for 7 days, twice a day (at 8:00 and at 20:00).”

• See source wording

  dogs · into a vein · daily

  Subchronic safety assessment of CIGB-500 in beagle dog after repeated daily dose administration over 28 days.Regulatory toxicology and pharmacology : RTP · 2025

  “All animals were allocated to four groups: Control, Low-Dose (300 μg/kg/day), Mid dose (1000 μg/kg/day) and High-Dose (2000 μg/μg/day).”

• 5 mg/kg

  rats · nasal spray

  Intranasal Delivery of a Ghrelin Mimetic Engages the Brain Ghrelin Signaling System in Mice.Endocrinology · 2025

  “Only GHRP-6 (5 mg/kg) increased food intake without adverse effects, prompting detailed analysis of meal patterns, neuronal activation in the arcuate nucleus (via Fos mapping) and neurochemical identification of c-fos messenger RNA (mRNA)-expressing neurons using RNAscope.”

• 300 nmol/kg

  mice · daily · for 9 weeks

  Atheroprotective and atheroregressive potential of azapeptide derivatives of GHRP-6 as selective CD36 ligands in apolipoprotein E-deficient mice.Atherosclerosis · 2020

  “Methods From 4 to 19 weeks of age, male apoe -/- mice were fed a high fat high cholesterol (HFHC) diet, then switched to normal chow and treated daily with 300 nmol/kg of MPE-001 ([aza-Tyr 4 ]-GHRP-6) or MPE-003 ([aza-(N,N-diallylaminobut-2-ynyl)Gly 4 ]-GHRP-6) for 9 weeks.”

• 9 mg/kg

  rats · for 5 days

  Inhibition of Ghrelin Activity by Receptor Antagonist [d-Lys-3] GHRP-6 Attenuates Alcohol-Induced Hepatic Steatosis by Regulating Hepatic Lipid Metabolism.Biomolecules · 2019

  “At 6 weeks, a subset of rats in each group were injected with either saline or ghrelin receptor antagonist, [d-Lys-3] GHRP-6 (DLys; 9 mg/kg body weight) for 5 days and all rats were sacrificed 2 days later.”

• 280 g

  rats ·

  Local injection of d-lys-3-GHRP-6 in the rat amygdala, dentate gyrus or ventral tegmental area impairs memory consolidation.Neuropeptides · 2018

  “Adult male Wistar rats weighing 230-280g were used.”

• 200 nmol

  mice ·

  Long-term treatment with the ghrelin receptor antagonist [d-Lys3]-GHRP-6 does not improve glucose homeostasis in nonobese diabetic MKR mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2018

  “Our results showed that [d-Lys3]-GHRP-6 (200 nmol/mouse) reduced pulsatile growth hormone secretion and body fat mass as expected but worsened glucose and insulin intolerances and increased cumulative food intake unexpectedly.”

• See source wording

  mice ·

  The effects of ghrelin antagonists [D-Lys(3) ]-GHRP-6 or JMV2959 on ethanol, water, and food intake in C57BL/6J mice.Alcoholism, clinical and experimental research · 2014

  “Three experiments tested ghrelin antagonism using different doses; experiment 1 tested 12 mg/kg JMV2959; experiment 2 tested 15 mg/kg DLys; experiment 3 tested 9 mg/kg JMV2959.”

• See source wording

  fish · by mouth

  Oral salmon acylated ghrelin increases food intake in common carp (Cyprinus carpio) via ghrelin receptors, likely through sensory nerves rather than systemic absorption.Peptides · 2026

  “Experimental diets containing sAG (ranging from 0.001 to 13.04 µg/g) were prepared and fed to carp at 2.0 % of body weight (BW) in single-shot trials.”

• See source wording

  chickens ·

  Effect of intracerebroventricular (ICV) injection of antimicrobial peptide expressed in the body-2 (LEAP-2) and its interaction with cannabinoid and ghrelin systems on food intake in broiler chickens.Poultry science · 2026

  “In experiment 1, chicken injected with group 1 ICV injection of saline, group 2 with LEAP2 (0.75 nmol), group 3 with LEAP2 (1.5 nmol) and group 4 with LEAP2 (3 nmol).”

• See source wording

  fish · another route

  The Ghrelin Analog GHRP-6, Delivered Through Aquafeeds, Modulates the Endocrine and Immune Responses of <i>Sparus aurata</i> Following IFA Treatment.Biology · 2025

  “In this study, the effects of the ghrelin analog GHRP-6 peptide included in the diet (500 µg/kg of feed) on the endocrine and immune responses of Sparus aurata following Incomplete Freund's adjuvant (IFA) treatment were assessed.”

• 6 mg/kg

  pigs · into a vein

  Inhibition of ghrelin activity by the receptor antagonist [D-Lys3]-GHRP-6 enhances hepatic fatty acid oxidation and gluconeogenesis in a growing pig model.Peptides · 2023

  “Growing pigs were intravenously injected with ghrelin receptor antagonist [D-Lys3]-GHRP-6 (DLys; 6 mg/kg body weight) for seven days to determine whether ghrelin plays a role in glucose and lipid metabolism.”